IROA® Biochemical Quantitation Kits

An innovative technology termed isotopic ratio outlier analysis (IROA®) was developed to overcome the analytical challenges associated with current, stable isotope-labelled compounds used in untargeted/targeted metabolic profiling. These challenges minimally pertain to analytical variance, artefactual peaks, and metabolite identification. The IROA® method uses metabolic incorporation of heavy (95% ¹³C) and light (5% ¹³C) nutrients, such as D-glucose and other carbon energy sources, to give rise to unique labelling patterns that can be readily identified and distinguished by mass spectrometry. Such a method would be useful in interlaboratory quantitative evaluations, if widely available.

Cambridge Isotope Laboratories Inc. is proud to offer a series of IROA® Biochemical Quantitation Kits for metabolic profiling of various cell populations and biological samples.

These kits contain the reagents and tools necessary for the successful metabolic labelling, identification, and quantitation of metabolites in various cell populations. Refer to product list for details.

Kit Features and Benefits

• Eliminates technical and analytical variance → increases reliability
• Removes artefacts and noise → increases precision and determination of metabolome
• Reproducible identification of knowns/unknowns
• Accurate, relative quantitation
• Automated solution (via software) → saves time
• Easy statistical interpretation of sample results
• Broad applicability with experimental perturbations being user-defined

Can’t find something? Email us at sales@gossinst.co.uk or message our team via the contact page. We are always happy to answer any questions you may have regarding IROA® Biochemical Quantitation Kits and will be delighted to assist you in locating precisely what you need.