Biomolecular NMR

Biomolecular NMR & Stable Isotope Labelling

Biomolecular NMR spectroscopy provides detailed structural and dynamic information about proteins, RNA and DNA at atomic resolution. It enables researchers to study biomolecules individually or within complexes, supporting advances in:

  • Structural biology

  • Mechanistic enzymology

  • Membrane protein research

  • Ligand binding studies

  • Rational drug design

High-resolution biomolecular NMR is essential for understanding macromolecular interactions and biological function in solution and near-native environments.

Computer generated image of DNA double helix
Biomolecular NMR chromatography

Why Use NMR for Biomolecular Studies?

Nuclear Magnetic Resonance (NMR) spectroscopy allows researchers to analyse:

  • Protein folding and conformational dynamics

  • RNA and DNA structure

  • Protein–ligand interactions

  • Multi-protein complexes

  • Membrane proteins in lipid environments

Unlike crystallography, NMR can provide dynamic information and does not require crystallisation.

However, NMR sensitivity is inherently limited. As a result, biomolecules are commonly enriched with stable isotopes such as 13C and 15N, and often deuterium (2H) to:

  • Enhance signal detection

  • Simplify complex spectra

  • Improve relaxation properties

  • Enable multidimensional NMR experiments

Membrane proteins, in particular, often require isotopic enrichment due to their size and complexity.

Isotopic Labelling for Biomolecular NMR

Advances in stable isotope labelling strategies have significantly expanded the size and complexity of biomolecules that can be studied by NMR.

In partnership with Cambridge Isotope Laboratories (CIL), Goss Scientific supplies high-quality stable isotopes and reagents for:

  • 13C protein labelling

  • 15N protein labelling

  • Uniform and selective deuteration

  • Sparse 13C labelling

  • Amino acid-specific methyl labelling

  • Site-specific isotopic enrichment

These tools support modern NMR techniques including:

  • TROSY and methyl-TROSY

  • Solid-state MAS NMR

  • Perdeuterated protein studies

  • Hyperpolarisation approaches

Sub Catagories

in vivo Protein Expression

Stable isotope-labelled media and reagents for prokaryotic and eukaryotic protein expression systems, including:

  • 13C-glucose

  • 15N-ammonium salts

  • Deuterated growth media

  • BioExpress® formulations

Optimised for high-yield isotopic incorporation in bacterial and eukaryotic systems.

Go to In vivo Protein Expression

Sparse Labelling for Protein NMR

Specialised sparse 13C labelling reagents and media designed to reduce spectral crowding and improve resonance assignment in complex systems.

Ideal for large protein NMR studies.

Go to Sparse Labelling for Protein NMR

Methyl and Amino Acid Type Labelling

Selective labelling reagents including:

  • Alpha-keto acids

  • 13C-labelled amino acids

  • Methyl-labelled precursors

Widely used in methyl-TROSY experiments for high-molecular-weight proteins.

Go to Methyl and Amino Acid Type Labelling

Protein Standards

Stable isotope-labelled protein standards supplied in solution or other formats for:

  • Instrument calibration

  • Method validation

  • Structural verification

Go to Protein Standards

Other Biomolecular NMR

Including:

  • Deuterated buffers

  • 17O-labelled reagents

  • Reverse micelle systems

  • Specialised NMR additives

Go to Other Biomolecular NMR

in situ Protein Expression

Reagents and kits for cell-free isotopic labelling systems, enabling:

  • Rapid protein synthesis

  • Controlled labelling patterns

  • Flexible isotope incorporation

Go to in situ Protein Expression

Membrane Proteins

Specialised reagents including:

  • Deuterated lipids

  • Deuterated detergents

  • Membrane-mimetic systems

Designed to stabilise membrane proteins in NMR-compatible environments.

Go to Membrane Proteins

Nucleic Acid Synthesis

Stable isotope-labelled reagents for RNA and DNA synthesis:

  • rNTPs

  • dNTPs

  • Deoxyphosphoramidites

Essential for isotope-enriched RNA and DNA NMR studies.

Go to Nucleic Acid Synthesis

Hyperpolarisation/MRI/MRS

13C-labelled substrates for:

  • Dynamic Nuclear Polarisation (DNP)

  • PHIP (Parahydrogen Induced Polarisation)

  • MRI and MRS research

These reagents support enhanced signal sensitivity in metabolic imaging applications.

Go to Hyperpolarisation / MRI / MRS

Stable Isotopes for Biomolecular NMR Catalogue

Download our comprehensive catalogue covering:

  • 13C and 15N labelling reagents

  • Deuterated compounds

  • Protein expression media

  • Nucleic acid synthesis reagents

  • Hyperpolarisation substrates

Download PDF

Why Choose Goss Scientific?

  • Official UK supplier of Cambridge Isotope Laboratories

  • Extensive biomolecular NMR reagent portfolio

  • High isotopic enrichment and purity standards

  • Technical expertise in isotope labelling strategies

  • Reliable UK distribution to research institutions

We support academic research groups, pharmaceutical R&D teams and structural biology laboratories across the UK.

Can’t find something? Email us at sales@gossinst.co.uk or message our team via the contact page. We are always happy to answer any questions you may have regarding Biomolecular NMR and will be delighted to assist you in locating precisely what you need.