INLIGHT® Kit

INLIGHT® was developed by David Muddiman and colleagues at North Carolina State University. This kit is designed for the relative quantification of enzymatically cleaved N-glycans in binary samples (i.e., case and control) by MS. The kit contains the following:

• ¹³C₆-labeled and an unlabelled biphenyl reagent (4-phenethylbenzohydrazide, phenyl 2-GPN) for hydrazide tagging of free glycans*
• Unlabelled maltoheptaose oligosaccharide (for quality control of extraction, derivatization, and LC-MS)
• Detailed instruction manual for performing the INLIGHT® strategy (see data file and data report below for maltoheptaose and fetuin)

*The phenyl 2-GPN reagent offers significant advantages (e.g., rapid and efficient derivatization, enhanced hydrophobicity and ionization efficiency; see references below). This facilitates the relative quantification of typically polar glycans by reversed-phase LC-MS.