Bile Acid Mixtures

High-Quality Bile Acid Standards for Targeted and Untargeted Analysis

Bile acids (BAs) are steroid-derived molecules that play a critical role in lipid digestion, cholesterol metabolism, and metabolic signalling pathways. Found predominantly in mammalian bile, bile acids function as biological surfactants, forming micelles that enable the absorption of dietary fats and fat-soluble nutrients.

Beyond digestion, bile acids regulate key physiological processes including:

• Glucose metabolism

• Energy homeostasis

• Cholesterol balance

• Gut microbiome signalling

• Hormone-like receptor activation

Goss Scientific, in partnership with Cambridge Isotope Laboratories (CIL), supplies high-purity bile acid mixtures designed specifically for LC-MS method development, validation, and quantitative metabolomics workflows.

These dried-down bile acid standards are available in two formats:

Unconjugated Bile Acid Mix

• Labelled: MSK-BA1-1

• Unlabelled: MSK-BA1-US-1

Conjugated Bile Acid Mix

• Labelled: MSK-BA2-1

• Unlabelled: MSK-BA2-US-1

Each mix is suitable for:

• Targeted LC-MS bile acid quantification

• Untargeted metabolomics screening

• Method development & optimisation

• Internal standard calibration

• Quality control & assay validation

The availability of stable isotope-labelled bile acids ensures accurate quantification, improved reproducibility, and robust analytical performance in complex biological matrices.

Our bile acid standards are widely used in research areas including:

• Liver disease and cholestasis studies

• Microbiome-bile acid interactions

• Metabolic syndrome research

• Pharmaceutical development

• Translational and clinical metabolomics

Application Note

Horvath, T.D.; Engevik, A.C.; Percy, A.J. (2022)
Bile acid analysis in mouse samples to study liver cholestasis etiology.

This study demonstrates the importance of reliable bile acid standards in developing robust LC-MS workflows for biological sample analysis.

Researchers have successfully used these bile acid mixtures in the development of a cost-effective bile acid sampling technique by repurposing dried blood spot (DBS) devices.

Read Goss Scientific’s review of this innovative DBS method to learn how bile acid quantification can be streamlined for translational and clinical applications.