Metabolomics Mixtures and Kits
Metabolomics is an increasingly important and growing area of research. The use of stable isotopes (as
internal standards) in combination with mass spectrometry allows researchers to identify and quantify metabolites in a given biological sample. This information is used to understand the physiology of an organism, its response to a drug, a change in its environment, and/or other external stimuli. It is also used to unravel disease mechanisms and to assess putative biomarkers.
Toward such initiatives, we offer a library of stable isotope-labelled standards (spanning a multitude of metabolic classes and pathways) that are available individually and/or as multi-component mixes or kits. These mixes and kits are designed to aid ease of use in untargeted or targeted metabolomics (for quality control, qualification, and/or quantitation). The mixtures are offered neat or as solutions, while the kits are additionally supplied with a user manual. The manuals outline general procedures and processing examples, as well as alternate method suggestions and data analysis guides for user reference.
Product Areas
CIL offers an amino acid mixture for use in standardization in untargeted and/or targeted metabolite quantification experiments
Using E. coli as a representative complex biological sample, CIL is pleased to offer Credentialed E. coli Cell Extract Kits for untargeted metabolomic developments.
To expedite method implementation and decrease laboratory downtime/costs, Waters has developed a series of optimized UPLC-MRM/MS methods for a variety of biologically relevant analytes
Quality control (QC) of methods and systems is necessary toward the generation of reliable mass spectrometry (MS) data.
The tricarboxylic acid (TCA) cycle, also known as the Krebs or citric acid cycle, plays an essential role in central carbon and energy metabolism. The study of its intermediates, and its ancillary compounds, has proven pivotal in not only understanding their impact on metabolism but also in profiling their function in oncogenesis, inflammation, and other pathologies.
Bile acids (BAs) are steroid-like compounds that act as a detergent in the breakdown of fats. This family of compounds comprises primary BAs (synthesized in the liver) and secondary BAs (produced in the gut by bacteria following modification of the primary BAs)
The yeast extracts — U-13C (ISO1) and unlabeled (ISO1-UNL) — are designed for internal standardization (i.e., matrix spike-in) quantitation experiments and for quality control evaluations in untargeted and targeted metabolomics.
Organic acids (OAs) play essential roles in energy metabolism pathways (e.g., glycolysis, tricarboxylic acid cycle), with the short-chained OAs emerging as important regulators of host immune response and transcriptional regulation.
To ensure high-quality metabolomics results, the method and instrument system must be qualified as being fit for purpose. This involves testing for losses or errors in the analytical workflow.