Metabolic incorporation of heavy isotopes into a proteome, as in SILAC and SILAM, is a popular method to prepare an internal standard or labelled control; however, some organisms and animals are not amenable to metabolic incorporation. Fortunately, analytes may be readily modified through chemical tagging reactions. Examples include the reductive animation of primary amines in proteins or peptides and hydrazide tagging of free N-linked glycans in proteomic samples. Since tagging reagents are compatible with many biological sample types, CIL is delighted to offer a collection of reductive methylation reagents and a glycan-tagging kit termed INLIGHT® (Individuality Normalization when Labelling with Isotopic Glycan Hydrazide Tag) for 2- or 3-plex relative quantitation. Each technique is described and referenced on their associated page.
Product Areas
INLIGHT® was developed by David Muddiman and colleagues at North Carolina State University. This kit is designed for the relative quantification of enzymatically cleaved N-glycans in binary samples (i.e., case and control) by MS.
The dimethyl labeling technique uses a reagent mixture (i.e., cyanoborohydride and formaldehyde in their unlabeled and stable isotope-labeled forms) to tag primary amines (i.e., the N-terminus and the ε-amino group of lysine) in proteins or peptides.