Stable isotope-labelled cellular biomass can be used in both proteomic and metabolomic investigations. In addition, quantitative, proteomic MS-based studies can benefit greatly from the use of purified, labelled intact protein as internal standards. The use of properly folded, labelled intact proteins are ideal internal standards because they will mimic, as close as possible, the physical and chemical properties of the target endogenous protein in a sample prior to, during and after digestion. In particular, they will undergo a similar degree of proteolytic cleavage as the unlabelled counterpart, thus improving the accuracy of the isotope dilution mass spectrometry (IDMS) experimental result for both middle-down or bottom-up methodologies.
CIL is pleased to offer labelled cell growth media for E. coli, insect cells, yeast, and eukaryotic cells. Specific human proteins may be overexpressed in a variety of cell types using these media in conjunction with recombinant techniques so that one can obtain a relatively large amount of labelled purified protein for proteomic studies.
CIL is also pleased to offer reagents and kits from CellFree Sciences (CFS), which are used to produce uniform or selectively labelled proteins in yields ideal for MS-based proteomic applications.
Product Information and Resources
- Stable Isotope-Labeled Peptide and Protein Reagents/Kits
- Full-Length Expressed Stable Isotope-Labeled Proteins for Quantification (Application Note 40)
- Insect Cell Media
- Mammalian Cell Media
- Top Ten Tips for Producing 13C, 15N Protein in Abundance (Application Note 15)
- CFS Products
- Premium PLUS Expression Kit for MS
- Yeast Media and Reagents
- Rich E. coli Media
