Membrane proteins make up between 20% and 30% of all expressed proteins and are medically important, as they represent more than half of all present-day drug targets. Membrane proteins have hydrophobic exteriors, are relatively dynamic, and are produced at relatively low levels. These factors create difficulties in obtaining stable preparations for NMR analyses. Despite the significant functional importance of membrane proteins, determining structures for these proteins is much more challenging than for globular proteins. Fortunately, there are many sample preparation conditions that can be chosen for solution and solid state NMR. Most preparations require a detergent or phospholipid for proper solubilization and stability. In this regard, Cambridge Isotope Laboratories is pleased to be able to offer deuterated phospholipids and detergents from FB Reagents, Ltd., a trusted source for deuterated lipids.
The study of structure, dynamics, and small molecule binding properties of membrane proteins are of great interest within the field of Structural Biology and pharmaceutical drug development. The study of membrane proteins is made challenging because only certain phospholipids and detergents can be used to ensure the protein assumes the correct three dimensional fold and remains active. Cambridge Isotope Laboratories, Inc. is proud to offer deuterated phospholipids and detergents manufactured by FB Reagents. FB Reagents is a trusted source of high quality deuterated phospholipids and detergents used in NMR-based scientific research.
Deuterated Phospholipids
- Simplification of 1H-NMR spectrum
- Easier detection of signals from biomolecules in multidimensional NMR experiments
- Minimization of dipolar relaxation effects leads to signal enhancement in certain systems
| Item No. | Description | Acronym | Size |
| DLM-11085 |
1,2-Dihexanoyl-sn-glycero-3-phosphocholine
(hexanoyl-D22, 97%; 50-60% on alpha carbons)
|
DH6PC-d22 | 100 mg |
| DLM-11092 |
1,2-Diheptanoyl-sn-glycero-3-phosphocholine
(heptanoyl-D26, 97%; 50-60% on alpha carbons)
|
DH7PC-d26 | 100 mg |
| DLM-11094 |
1,2-Dipalmitoyl-sn-glycero-3-phosphocholine
(dipalmitoyl-D62, 97%; 50-60% on alpha carbons)
|
DPPC-d62 | 100 mg |
| DLM-11095 |
1,2-Dioleoyl-sn-glycero-3-phosphocholine
(dioleoyl-D64, 97%; 50-60% on alpha, vinyl carbons)
|
DOPC-d64 | 50 mg |
| DLM-11096 |
1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
(fatty acids-D63, 97%; 50-60% on alpha, vinyl carbons)
|
POPC-d63 | 50 mg |
| DLM-11097 |
1,2-Dimyristoyl-sn-glycero-3-phosphoglycerol, NH4+
(dimyristoyl-D54, 97%; 50-60% on alpha carbons)
|
DMPG-d54 | 100 mg |
| DLM-11098 |
1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine
(dipalmitoyl-D62, 97%; 50-60% at alpha carbon)
|
DPPE-d62 | 100 mg |
| DLM-11099 |
1,2-Dipalmitoyl-sn-glycero-3-phosphoserine, NH4+
(dipalmitoyl-D62, 97%; 50-60% on alpha carbons)
|
DPPS-d62 | 50 mg |
| DLM-11100 |
1-Myristoyl-2-lyso-sn-glycero-3-phosphoglycerol NH4+
(myristoyl-D27, 97%; 50-60% at alpha carbon)
|
LMPG-d27 | 100 mg |
| DLM-11101 |
1-Palmitoyl-2-lyso-sn-glycero-3-phosphoglycerol NH4+
(palmitoyl-D31, 97%; 50-60% at alpha carbon)
|
LPPG-d31 | 100 mg |
| DLM-11102 |
Lauryl-N,N-dimethyl N-oxide
(lauryl-D25; dimethylamine-D6, 97%) |
LDAO-d31 | 100 mg |
| DLM-11103 | Dodecyl-β-D-maltopyranoside (dodecyl-D25, 97%) | DDM-d25 | 100 mg |
| DLM-11104 |
Decanoyl-rac-glycerol
(decanoyl-D19, glycerol-D5, 97% (50-60% on alpha)
|
10-MAG-d24 | 100 mg |
| DLM-11105 | L-α-Glycerophosphocholine-d9 (methyl-D9, 97%) | GPC-d9 | 100 mg |
References
Movelan, K.T.; Wegstroth, M.; Overkamp, K.; et al. 2020. Imidazole-imidazole hydrogen bonding in the pH-densing histidine side chains of influenza A M2. J Am Chem Soc, 142(6), 2704-2708. PMID: 31970979