The dimethyl labelling technique uses a reagent mixture (i.e., cyanoborohydride and formaldehyde in their unlabelled and stable isotope-labelled forms) to tag primary amines (i.e., the N-terminus and the ε-amino group of lysine) in proteins or peptides. This is a fast, straightforward, and inexpensive approach to conduct 2- or 3-plex quantitative proteomic analyses of a variety of sample types (e.g., lysate, tissue). To facilitate such experiments (example provided in Application Note 38), CIL is pleased to offer a variety of reductive methylation reagents.
