SILAM

SILAM refers to labelling an entire rodent with heavy stable isotopes for quantitative proteomic tissue analysis. Labelling an entire proteome with heavy isotopes in vivo generates an ideal standard for quantitative proteomics. When a heavy labelled proteome is mixed with an unlabelled proteome then digested, every unlabelled peptide identified by the mass spectrometer can be quantified by its corresponding heavy peptide.

The advantage of metabolic labelling over in vitro tagging techniques is that the heavy and unlabelled samples are mixed before sample preparation, preventing variability between preparations from distorting the quantification results. This is especially important when extensive sample preparation (e.g. isolation of an organelle) is required.

In SILAM, the rodent food is altered to contain heavy lysine or ¹⁵N spirulina as the only protein source. The heavy tissues are used as internal standards for quantitative proteomic analysis of basic mammalian physiology and animal models of disease.

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