Metabolic Labelling
One type of proteome labelling introduces a stable isotope amino acid(s) to cell growth media or rodent feed. Growth and feeding periods allow the stable isotope-labelled amino acid(s) to become metabolically incorporated into the proteome. Experiments involving cell culture are referred to as SILAC (stable isotope labelling by amino acids in cell culture), while mammalian systems are referred to as SILAM (stable isotope labelling in mammals). Each metabolic labelling technique is described and referenced on their associated page.
Product Areas
SILAC refers to labelling cultured cells with heavy amino acids for quantitative proteomic analysis. Labeling an entire proteome with heavy amino acids in vivo generates an ideal standard for quantitative proteomics.
SILAM refers to labelling an entire rodent with heavy isotopes for quantitative proteomic tissue analysis.
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