Maltose Binding Protein (NEX-MBP)

Maltose Binding Protein (NEX-MBP) is a 44.9 kDa monomeric protein for which multiple sets of resonance assignments (BMRB database) and 3D structures (PDB database) are publicly available. This product is uniformly ²H,¹⁵N,¹³C-enriched with selective incorporation of protons into methyl groups of Ile-δ1, Leu-δ and Val-γ side chains. As nonuniform sampling (NUS) and other NMR techniques emerge to push the size limitations of NMR to new boundaries, large protein standards, such as NEX-MBP, will be required to test data-collection and processing strategies.

NEX-MBP sample formulations:

NEX-MBP1: Apo conformation 0.5 mM 2 H,¹⁵N,¹³C and ILV methyl ¹H,¹³C MBP in 10% D₂O, 0.02% NaN₃ , 20 mM sodium phosphate @ pH 7.2
NEX-MBP2: Closed conformation 0.5 mM 2 H,¹⁵N,¹³C and ILV methyl 1 H,¹³C MBP with 3 mM maltotriose, 10% D₂O, 0.02% NaN₃ , 20 mM sodium phosphate @ pH 7.2
NEX-MBP3: Open conformation 0.5 mM 2 H,¹⁵N,¹³C and ILV methyl 1 H,¹³C MBP with 2 mM β-cyclodextrin, 10% D₂O, 0.02% NaN₃ , 20 mM sodium phosphate @ pH 7.2

E.coli Maltose Binding Protein (27-396), Apo Conformation

NEX-MBP1-U unlabelled

NEX-MBP1-N (¹⁵N, 95%)

NEX-MBPI-CN-5 (¹³C, 5%; ¹⁵N, 95%)

NEX-MBP1-CN (¹³C, 95%; ¹⁵N, 95%)

NEX-MBP1-CDN (¹³C, 95%; D, 95%; ¹⁵N, 95%)

NEX-MBP1-ILV (¹³C, 95%; D, 95%; ¹⁵N, 95%; ¹³CH₃ -ILV)

NEX-MBP1-ILVFY (¹³C, 95%; D, 95%; ¹⁵N, 95%; ¹³CH₃ -ILVFY)

E.coli Maltose Binding Protein (27-396), Closed Conformation

NEX-MBP2-U unlabelled

NEX-MBP2-N (¹⁵N, 95%)

NEX-MBP2-CN-5 (¹³C, 5%; ¹⁵N, 95%)

NEX-MBP2-CN (¹³C, 95%; ¹⁵N, 95%)

NEX-MBP2-CDN (¹³C, 95%; D, 95%; ¹⁵N, 95%)

NEX-MBP2-ILV (¹³C, 95%; D, 95%; ¹⁵N, 95%; ¹³CCH₃-ILV)

NEX-MBP2-ILVFY (¹³C, 95%; D, 95%; 15N, 95%; 13CH3-ILVFY)

E.coli Maltose Binding Protein (27-396), Open Conformation

NEX-MBP3-U unlabelled

NEX-MBP3-N (¹⁵N, 95%)

NEX-MBP3-CN-5 (¹³C, 5%; ¹⁵N, 95%)

NEX-MBP3-CN (¹³C, 95%; ¹⁵N, 95%)

NEX-MBP3-CDN (¹³C, 95%; D, 95%; ¹⁵N, 95%)

NEX-MBP3-ILV (¹³C, 95%; D, 95%; ¹⁵N, 95%; ¹³CH₃-ILV)

NEX-MBP3-ILVFY (¹³C, 95%; D, 95%; ¹⁵N, 95%; ¹³CH₃-ILVFY)

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