Credentialed E. coli Cell Extract Kits
Introduction to Credentialed E. coli Cell Extract Kits
Metabolomics generates large, complex datasets. Due to this complexity, it is challenging to assess method
performance, to identify analytes, and to compare data across laboratories and experiments. Typically, the diversity of metabolite analytes and metabolomic study designs precludes the use of one or a few standards to address these challenging questions. A solution, developed by Patti and colleagues, is the credentialing protocol. This utilizes E. coli cell extracts and novel Credentialing software to discriminate features of biological origin from potential contaminants. For example, these could include plastic leachable, solvent impurities and carryover. Furthermore, artifacts or informatic errors can be discerned without having to identify metabolite structures.
CIL is pleased to offer Credentialed E. coli Cell Extract Kits for untargeted metabolomic developments, which use E. coli as a representative complex biological sample. These kits are supplied as a solution, MSK-CRED-KIT, and as dried down, MSK-CRED-DD-KIT. As such, are designed to assist in optimizing methods for untargeted, metabolomic profiling studies (see references below for example applications).
Contents
- 13C-labeled E. coli cell extract (dried down or 100 µL).
- Unlabelled E. coli cell extract (dried down or 100 µL).
- Document package (supplied via QR code).
Package includes user manual, which contains instructions on solution/sample preparation and data analysis.
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