Application Notes

Contents

Go to Proteomics Application Notes

Chemical Labelling

Chemical Tagging

Metabolic Labelling

Protein Expression

QC and Quantitation Kits

Go to Biomolecular NMR Application Notes

Hyperpolarisation/MRI/MRS

in situ Protein Expression

in vivo Protein Expression

Membrane Proteins

Methyl and Amino Acid Type Labelling

Selective Labelling

Go to Environmental Application Notes

Ecotoxicology and Exposure Analysis

Proteomics

Application Notes: Chemical Labelling

Synthesis to Analysis: Stable Isotope- Labelled Peptides for Proteomic Applications

Application Notes: Chemical Tagging

Stable Isotope Dimethyl Labelling

Individually Normalisation when Labelling with Isotopic Glycan Hydrazide Tags (INSIGHT™): A Novel N-Linked Glycan Relative Quantification Strategy

The Use of Adenosine 5'-Triphosphate (γ-P18O4, 97%) for the Unambiguous Identification of Phosphopeptides

Selection and Synthesis to Analysis: Stable Isotope- Labelled Peptides for Proteomic Applications

Application Notes: Metabolic Labelling

Metabolic Isotopic Analysis Reveals Mitochondrial Loss of Function in pRb- Deficient Cells in vivo

Analysis of Whole-Body Branched-Chain Amino Acid Metabolism in Mice Utilising 20% Leucine 13C6 and 20% Valine 13C5 Mouse Feed

Analysis of Tyrosine Kinase Signalling in Human Cancer by Stable Isotope Labelling with Heavy Amino Acids in Mouse Xenografts Utilising Mouse Express® Lysine 13C6 Mouse Feed

Targeted LC-SRM/MS Quantification of Mammalian Synaptic Proteins with Mouse Express® Brain Tissue, a New Isotopically Labelled Proteome Standard

Stable Isotope Labelling in Mammals with 15N Spirulina

Metabolic Incorporation of Stable Isotope Labels into Glycans

Application Notes: Protein Expression

Full-Length Expressed Stable Isotope- Labelled Proteins for Quantification

Application Notes: QC and Quantitation Kits

Translation and Implementation of PeptiQuant™ Plus Human Plasma BAK-270

Lipidomics and Metabolics

Application Notes: Lipidomics

Targeted MRM Screening of U-13C Lipid Yeast Extracts for Robust Lipidomics Applications

Application Notes: Metabolism

Fluxing Through Cancer: Tracking the Fate of 13C-Labelled Energy Sources Glucose and Glutamine in Cancer Cells and Mouse Tumours

Tracing Lipid Disposition in vivo Using Stable Isotope- Labelled Fatty Acids and Mass Spectrometry

Determination of Nitric Oxide Production and de novo Arginine Production with Stable Isotopes

Determining Protein Turnover in Fish with D7-Leucine

Application Notes: Metabolomics

Bile Acid Analysis in Mouse Samples to Study Liver Cholestasis Etiology

From QC to Quantitation: Utility of QReSS™ Metabolites in FBS Measurements

Standardising Quantitative Metabolomics Analyses Through the QReSS™ Kit

Organic Acid Quantitation in Mouse Muscle by Ion Chromatography-Mass Spectrometry with Isotopically Labelled Standards

Pathway Targeted Metabolic Analysis in Oral-Head and Neck Cancer Cells Using IC-MS

Biomolecular NMR

Application Notes: Hyperpolarisation/MRI/MRS

Development of Hyperpolarised Metabolic Contrast Agents Using PASADENA

Application Notes: in situ Protein Expression

Cell-Free Protein Synthesis with 2H/15N/13C-Labelled Amino Acids in H2O for the Production of Perdeuterated Proteins with 1H in the Exchangeable Positions

Application Notes: in vivo Protein Expression

[2,3-13C]- Labelled Aromatic Residues as a Means to Improving Signal Intensities and Kick-Starting the Assignment of Membrane Proteins by Solid-State MAS-NMR

Effective Site Specific Isotopic Labelling (15N, 13C Glycine, 15N, 13C Phenylalanine; 15N Tryptophan) Expression Optimisation Using BioExpress® 2000 Media

in vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies

Top Ten Tips for Producing 13C, 15N Protein in Abundance

Efficient Uniform Isotope Labelling of Proteins Expressed in Baculovirus-Infected Insect Cells Using BioExpress® 2000

Optimisation of BioExpress® Supplimentation of M9 Cultures

Application Notes: Membrane Proteins

Uniform Isotope Labelling of Eukaryotic Protein in Methylotrophic Yeast for High-Resolution NMR Studies - Extension to Membrane Proteins

Application Notes: Methyl and Amino Acid Type Labelling

Stereospecific Leu/Val Methyl Labelling: An Important Technology for NMR Studies of High-Molecular-Weight Complexes

Specific Isotopic Labelling of Methyl Groups Has Extended the Molecular Weight Limits for NMR Studies of Protein Structure and Dynamics

Application Notes: Selective Labelling

Production of U-[2H], Thyr-γ2[13CH3] Labelled Proteins for Methyl-TROSY NMR

Environmental

Application Notes: Ecotoxicology and Exposure Analysis

Human Biomonitoring: Attogram Level Sensitivity and Consequences for Analytical Standards Purity