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Application Notes - Stable Isotopes

Please contact Goss Scientific for pricing and availability of any products used in the application notes below.

Goss Scientific are UK distributors for Cambridge Isotope Laboratories range of stable isotopes.


Metabolic Labeling

Metabolic Isotopic Analysis Reveals Mitochondrial Loss of Function in pRb-Deficient Cells in vivo

Application Note 43

Targeted LC-SRM/MS Quantification of Mammalian Synaptic Proteins with Mouse Express® Brain Tissue

Application Note 32

Stable Isotope Labeling in Mammals with 15N Spirulina

Application Note 24

Stable Isotope Labeling in Proteomics

Application Note 11



Chemical Tagging

Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT™)

Application Note 37

The Use of Adenosine 5'-Triphosphate for the Unambiguous Identification of Phosphopeptides

Application Note 17

Specific Isotopic Labeling of Methyl Groups Has Extended the Molecular Weight Limits for NMR Studies of Protein Structure and Dynamics

Application Note 16

Protein Expression

 Full-Length Expressed Stable Isotope-Labeled Proteins for Quantification

Application Note 40

Stable Isotope Dimethyl Labeling

Application Note 38

 Heavy Labeled MS Protein Standards

Stable Isotope-Labeled ApoA-1 as a Global Standard for Quantitative Proteomics Studies

Application Note 46


Metabolic Research



Metabolic Isotopic Analysis Reveals Mitochondrial Loss of Function in pRb-Deficient Cells in vivo

Application Note 45

Fluxing Through Cancer: Tracking the Fate of 13C-Labeled Energy Sources Glucose and Glutamine in Cancer Cells and Mouse Tumors

Application Note 34

Tracing Lipid Disposition in vivo Using Stable Isotope-Labeled Fatty Acids and Mass Spectrometry

Application Note 31

Determination of Nitric Oxide Production and de novo Arginine Production with Stable Isotopes

Application Note 30

Determining Protein Turnover in Fish with D7-Leucine

Application Note 29 

Metabolic Incorporation of Stable Isotope Labels into Glycans

Application Note 23




Thermo-CIL App Note Pathway Targeted Metabolomic Analysis in Oral-Head and Neck Cancer Cells Using IC-MS

Application Note 44



MS/MS Standards

NSK-A-TS and NSK-B-TS Instructions for Use

Application Note 41


Biomolecular NMR


In situ Protein Expression


Cell-Free Protein Synthesis with 2H/15N/13C-Labeled Amino Acids in H2O for the Production of Perdeuterated Proteins with 1H in the Exchangeable Positions

Application Note 36



In vivo Protein Expression


Isotope Labeling of Alanine Methyl Groups on a Deuterated Background for NMR Studies of High-Molecular-Weight Proteins

Application Note 25

[2,3-13C]-Labeled Aromatic Residues as a Means to Improving Signal Intensities and Kick-Starting the Assignment of Membrane Proteins by Solid-State MAS-NMR

Application Note 22

Effective Site Specific Isotopic Labeling 15N, 13C Glycine; 15N, 13C Phenylalanine; 15N Tryptophan) Expression Optimization Using BioExpress® 2000 Media

Application Note 20

In vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies

Application Note 19

Top Ten Tips for Producing 13C, 15N Protein in Abundance

Application Note 15 

Efficient Uniform Isotope Labeling of Proteins Expressed in Baculovirus-Infected Insect Cells Using BioExpress® 2000

Application Note 14

Optimization of BioExpress Supplementation of M9 Cultures

Application Note 12


Stereospecific Leucine/Valine Methyl Labelling

Stereospecific Leucine / Valine Methyl Labelling

Application Note 48

Membrane Proteins

Uniform Isotope Labeling of Eukaryotic Proteins in Methylotrophic Yeast for High-Resolution NMR Studies – Extension to Membrane Proteins

Application Note 26



Selective Labeling


Production of U-[2H], Thyr-γ2[13CH3] Labeled Proteins for Methyl-TROSY NMR

Application Note 39





Development of Hyperpolarized Metabolic Contrast Agents Using PASADENA

Application Note 21



Other BioMolecular NMR


Using Stable Isotopes to Solve Problems in Combinatorial Chemistry

Application Note 1



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